Molecular marker detection of rice bacterial leaf blight resistant gene Xa23.

In the present study,Zhongye 5112(donor),Gui 649(acceptor) and their 35 hybridized F4 off-springs were detected with the PCR-based molecular marker RM206,which is closely linked to rice bacterial leaf blight(BLB) resistant gene Xa23.The results showed that the allele of Xa23 appeared in the Zhongye 5112 and its 13 F4 off-springs with rate of 37.14%,while it could not be detected in Gui 649 and other 22 off-springs.These results indicated that the heredity of gene Xa23 was stable and had strong inheritance probability during hybridization.The present study provides a good foundation for breeding good intermediate materials resistant to BLB and making hybrid rice combinations and conventional rice varieties with wide and stable resistance.