Precise Control of Gold Nanoparticles on DNA Origami for Logic Operation

Owing to their capacity for accurate structural control and complex programmability, DNA molecules have been extensively studied in relation to the construction of nanodevices. However, the existing logic gate sections based on DNA self-assembly were independent of each other, which hampered the development of large-scale integrated DNA circuits. Herein, we have explored a logic operation device with excellent scalability based on assembling and selectively releasing AuNPs on DNA origami, and have performed YES gate, OR gate, AND gate, and three-input composite gate. In the experiment, the logic operation result is detected by gel analysis and TEM image. The resolution of the output signals was greatly improved by determining the releasing of AuNPs from two-layer honeycomb origami. Our study provides a promising approach for building more complex large-scale DNA logic circuits.