T-Cell Recognition of HLA Class I Molecules

The purpose of the Class I T-Cell Clone component of the Tenth Workshop (10WS) was to determine the extent to which HLA class I molecules express polymorphisms recognizable by alloreactive or antigen-specific class I-restricted cloned T cells. A standard protocol for testing available proliferative and cytolytic T-cell clones for reactivity against the 10WS core cell line panel was developed in parallel to the Class II T-Cell Clone component, with the exception that monoclonal antibody blocking studies were not performed with the anti-class I T-cell clones. The same method for data reduction (whereby a specific proliferative or cytolytic activity was converted into simple numerical scores (“1”=negative; “6” or “8”=strong positive) was used.