Method for producing proenzyme of transglutaminase through fermentation

The invention discloses a method for producing proenzyme of transglutaminase through fermentation; mutation E62D-tag 1 of the proenzyme of the transglutaminase is taken as an original strain, aqua ammonia is used for controlling the pH value to be not less than 7, and in case of dissolved oxygen bounce, feeding is carried out through an index fed-batch manner; glycine and CaC12 are added and constant-speed flow feed liquid supplement is started until induced concentration is reached. After the adoption of the method, the yield of the proenzyme of the transglutaminase is greatly improved.