Purification of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) Esters from Squid Oil by Silver Ion Chromatography

EPA and DHA extracted from methyl esterified squid oil were purified by silver exchanged resin, silver nitrate-impregnated silica gel, silver exchanged zeolite, and silica gel column chromatography, among which columnchromatography using mixture of silver exchanged resin and silica gel (10% by weight) showed the best result.By this simple purification method, EPA and DHA were concentrated from 12.5 to 27.9% (yield, 86.0%) andfrom 21.7 to 49.5% (yield, 87.3%), respectively. Silver exchanged resin had additional advantages of outstandingreusability and simple recovery of silver.Key words: