Effects of Cobalt Chloride on Proliferation and Apoptosis of NIH3T3 Cells in Mice

Objective:To study effects of Cocl2 on survival,proliferation and apoptosis of mouse NIH3T3 cells.Methods:The survival,proliferation and apoptosis effects of different concentrations of Cocl2 on mouse NIH3T3 were detected by MTT and flow cytometer assay.The expression of HIF-1α,Bcl-2 and Bax protein were detected by Western blot.Results:(1) MTT assay showed that the cellular proliferation activity was inhibited significantly by higher concentration(≥500 μmol/L) of Cocl2,however lower concentration(250 μmol/L) of Cocl2 had little effects on the proliferation of NIH3T3.(2) Flow cytometer assay showed higher concentration of Cocl2 increased the early apoptosis and necrosis rates of NIH3T3,but the lower concentration of Cocl2 could not affect the apoptosis and necrosis rates.(3) The expression of HIF-1α protein could be upregulated by different concentrations of Cocl2 in NIH3T3 cells and the expression ratio of Bax/Bcl-2 was increased.Conclusions:Higher concentrations of Cocl2 could inhibit the proliferation and promote the apoptosis of mouse NIH3T3 cells.