Eradication of enterococci biofilms by lactic acid alone and combined with chlorhexidine and cetrimide
2012
Objective: The antimicrobial activity of lactic acid (LA) alone or in combination with chlorhexidine (CHX) and
cetrimide (CTR) against three
Enterococcus faecalis
strains,
E. faecalis
ATCC 29212,
E. faecalis
EF-
D1 and
E.
faecalis
U-1765, one
Enterococcus durans
strain and one dual-
species biofilm was investigated.
Study Design: The irrigating solutions tested were 20%, 15%, 10%, 5% and 2.5% LA, alone and in combination
with 2% CHX and with 0.2% CTR. The biofilms were grown in the MBEC
TM
high-throughput device for 24 hours
and exposed to the solutions for 30 seconds and 1 minute. “Eradication” was defined as 100% bacterial kill.
Results: Twenty percent LA eradicated all enterococci biofilms after 30 seconds contact time. The association of
LA + 0.2% CTR achieved better results than LA alone, in contrast with the results obtained using LA + 2% CHX.
E. durans
was eradicated by all the tested solutions at 1 minute. The dual-
species biofilm,
E. faecalis
ATCC 29212
+
E. durans,
gave intermediate values of the pure cultures.
Conclusions: LA is capable of eradicating enterococci biofilm at a concentration of 20%. The combination of
lower concentrations with 0.2% CTR achieved eradication after 1 minute.
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