Genetic variability among Syphacia obvelata isolates from laboratory mice in four different geographical locations of China revealed by sequence analyses of five mitochondrial genes

AbstractSyphacia obvelata is a rodent nematode with high prevalence in laboratory mice. In the present study, we examined the genetic variability of S. obvelata from naturally infected laboratory mice in four different provinces, China. Five mitochondrial (mt) DNA regions, namely cytochrome c oxidase subunit 1 (pcox1), cytochrome b (pcytb), large subunit ribosomal RNA (prrnL) and NADH dehydrogenase subunits 1 and 5 (pnad1 and pnad5), were amplified separately from individual nematodes by PCR, and then sequenced directly. The size of the sequences of pcox1, pcytb, prrnL, pnad1 and pnad5 was 628 bp, 555 bp, 548 bp, 548 bp and 561 bp, respectively. While the intra-specific sequence variations within S. obvelata were 0–1.0% for pcox1, 0–1.6% for pcytb, 0–2.8% for prrnL, 0–2.0% for pnad1 and 0–1.8% for pnad5, the inter-specific sequence differences among members of the Oxyuridae were significantly higher, being 14.0–17.5% for pcox1, 27.5–32.9% for pcytb, 35.8–37.2% for prrnL, 22.2–26.8% for pnad1 and 22.3–25.2...