ГИС: ВОЗМОЖНОСТИ АНАЛИЗА ДАННЫХ ФЕНО- И ГЕНОТИПИРОВАНИЯ ХОЛЕРНЫХ ВИБРИОНОВ 01 СЕРОГРУППЫ ЭЛЬ ТОР, ИЗОЛИРОВАННЫХ ИЗ ВОДНЫХ ОБЪЕКТОВ ОКРУЖАЮЩЕЙ СРЕДЫ НА ТЕРРИТОРИИ РОССИЙСКОЙ ФЕДЕРАЦИИ
2016
Aim. Application of the authors’ GIS «Cholera 1989-2014» for systematization of atoxigenic strains of serogroup 01 cholera vibrios (ctxAB-tcpA-, ctxAB-tcpA+), isolated from aquatic objects of the environment by pheno- and genotype. Materials and methods. A sample of 304 Vibrio cholerae 01 strains was studied. Isolation of 39 genes related to pathogenicity was carried out. Discrimination ability of a set of genes was determined by Simpson formula. Cluster analysis was carried out by UPGMA method. Results. Analysis of multi-year data on aquatic V. cholerae 01 strains in country’s subject was carried out using GIS. A possibility of systematization of phenotypes of the isolated strains by defined parameters was shown. An experimental program for detection of presence/lack of various genes and their combinations forgenotyping was developed. Conclusion. GIS was established to allow to carry out analysis of phenotypes by defined parameters, as well as implement approximate systematization of genotypes of atoxigenic strains of cholera vibrios 01 by optimally sufficient detection of 14 genes.
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