A potential use of a : Comparisons with 14c-labelled 2-deoxyglucose autoradiography and histopathological examination123: Comparisons with 14c-labelled 2-deoxyglucose autoradiography and histopathological examinationi-labelled benzodiazepine receptor antagonist as a predictor of neuronal cell viability: Comparisons with 14c-labelled 2-deoxyglucose autoradiography and histopathological examination

SUMMARY In the treatment and therapy of patients suffering a stroke, it is very important to predict whether viable neurones, even those of poor function, remain intact in the lesions of the brain. To determine whether viable neurones of low functional activity are represented in in vivo neuroreceptor imaging, we undertook experiments in gerbils with cerebral infarction, in which we examined histological changes and the results of dual-tracer in vivo autoradiography of glucose utilization with 14C-labelled deoxyglu-cose and benzodiazepine receptor binding with 123I-labelled Ro 16–0154. The unrelated findings of cerebral glucose metabolism and benzodiazepine receptor binding were observed in the primary infarct lesion and in remote areas, including the ipsilateral striatum and thalamus. SUMMARY Our experiments showed that when viable neurones with low functional activity remain intact, normal in vivo binding to benzodiazepine receptors is demonstrated as hypometabolism of glucose utilization. This functional, contrast-enhanced technique with 123I-labelled Ro 16–0154 may have an important role to play in the prediction of neuronal cell viability after recent brain infarction in experimental animals and humans using single photon emission tomography (SPET).