Use of 5-Nitroindole-2′-deoxyribose-5′-triphosphate for Labelling and Detection of Oligonucleotides†

Abstract The 5′-triphosphate of 5-nitroindole-2′-deoxyriboside has been shown to be a good substrate for terminal deoxynucleotidyl transferase (TdT). An antibody has been prepared for the detection of 5-nitroindole and has been used for the detection of 5-nitroindole tailed DNA both in single-stranded form and after hybridisation to a template. This is therefore a new method for the detection of nucleic acid probes. This paper is dedicated with great regard to the late Professor Tsujiaki Hata