A rapid detection method of inbred mouse DNA genetic quality based on PCR-LDR platform

Objrctive Purity of laboratory animals plays a critical role affecting the experimental results.The aim of this study was to validate a high-throughput genotyping platform,valuable in inbred mouse genetic DNA monitoring and strain identification.Methods Multiple polymerase chain reaction and ligase detection reaction(PCR-LDR) genotyping panels were constructed.Forty-five single-nucleotide polymorphism(SNP) on 21 chromosomes were selected as targets,as well as specific primers and probes to these SNP were designed,respectively.A multiple PCR-LDR genotyping protocol was established.Results Forty-five SNP were successfully genotyped in four panels.The positive detection rate of 43,44 and 45 SNPs were 100%,90.9% and 36.4%,respectively.All samples collected were homozygous and their genotypes were identified by PCR-LDR.Conclusion The results of this study provide a rapid and high-throughput genotyping approach,which is sufficient for genetic contamination monitoring and strain identification.