Human miR-328 antisense nucleic acid and application thereof

The invention discloses antisense oligonucleotide for suppressing expression of human microRNA-328, and application of the antisense oligonucleotide. The antisense oligonucleotide is specifically combined to the human miR-328, comprises sequences complementary with at least 13 continuous nucleotides in a 5'-CUGGCCCUCUCUGCCCUUCCGU-3' nucleotide sequence, particularly a 5'-ACGGAAGGGCAGAGAGGGCCAG-3' sequence. The antisense oligonucleotide can be ribonucleotide, deoxyribonucleotide or chimera of ribonucleotide and deoxyribonucleotide, can modify any nucleotide in a chain. The miR-328 antisense oligonucleotide can effectively suppress the expression of miR-328 in human glioma cells, and suppress the cells from growing and proliferating to effectively treat human glioma and other tumors with high miR-328 expression.