Enhancement of leaf rust resistance of Siberian winter wheat varieties by marker-assisted selection

Cultivation of winter wheat varieties in the West Siberian region of Russia has competitive advantages compared to spring varieties: utilization of spring-summer moisture, early maturation and harvest and a high yield potential. The poor resistance of winter varieties to foliar diseases results in significant yield losses and facilitates the spread of pathogens to the spring wheat cultivars. The present study was conducted to evaluate the effectiveness of molecular markers specific for VRN-1 and Lr loci in selecting winter wheat genotypes resistant to leaf rust. The winter wheat cultivars Biyskaya ozymaya and Filatovka were crossed with spring wheat introgression lines 21-4 and 5366-180 and the spring wheat cultivar Tulaikovskaya 10 carrying LrTt2, LrAsp5 and Lr6Ai#2 loci from Triticum timopheevii, Aegilops speltoides and Thynopyrum intermedium, respectively. To identify winter wheat plants homozygous for target loci, F2 populations were screened with functional markers to VRN-1 genes and with markers spe...