Changes of serum amino acid profile in guine a pigs with bile duct ligation

Liver plays a critical role in detoxification, synthesis and metabolism. Previous studies indicate that blood amino acid changes in severe liver disease. Glycine, a precursor of glutathione, exerts protective effects against free radicalinduced liver injury. This study aimed to establish a method measuring serum large neutral amino acids (LNAAs) concentrations with liquid chromatography-tandem mass spectrometry (LCMS/MS) and to examine the effects of bile duct ligation on serum liver function tests and amino acids concentrations. We further investigated whether treatment of glycine, alanine, serine or tyrosine would protect against bile duct ligationinduced damage.