Method for regenerating plants of Chinese rose by using leaves as explants

2010 
The invention belongs to the technical field of plant tissue and cell culture, and in particular relates to a plant regeneration method by using leaves of Chinese rose as explants through a somatic cell embryogenesis path. The method comprises the following three culture stages: (1) culturing the Chinese rose explants on an induction medium, and directly inducing somatic embryos from the explants, wherein the explants are wrapped leaves of Chinese rose respectively; (2) inoculating the inoculate on a propagation culture medium for propagation and accelerating further differentiation thereof; and (3) inoculating the somatic embryos subjected to propagation culture to a somatic embryo seedling culture medium and a rooting culture medium and regenerating complete plants. The method has the advantages of wide sources of explants, convenient material selection and the like. The prepared somatic embryos are agrobacterium-mediated excellent receptors through genetic transformation and can be used for research on genetic transformation of the Chinese rose. The invention also relates to components of culture media for the method.
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