Study on modern processing technologies of aconiti kusnezoffii radix

Objective: To compare different processing technologies of Aconiti Kusnezoffii Radix,and to optimize a kind of ″low toxicity and high efficiency″,simple and practical processing technology for Aconiti Kusnezoffii Radix.Methods: HPLC method was used to determine the content of six kinds of alkaloids,and titration method to determine the content of total alkaloids.These contents combined with production were used to evaluate the modern processing technologies of Aconiti Kusnezoffii Radix.Results: There was serious drain of Aconitum alkaloids after treated by soaking,and the loss rates of the content of monoester alkaloids,diester alkaloids and total alkaloid were 20.97%,31.13% and 14.57%,respectively.The content of alkaloids could maximize the retention using wetting method,the best methods for reducing poison were atmospheric pressure and high pressure steaming cooking methods,and its total diester alkaloids content was too low to be detected,the contents of alkaloids in samples of two kinds of high-temperature baking were 0.6903 mg /g and 0.5575 mg /g,which were higher than the requirement of Chinese Pharmacopoeia.The content of monoester alkaloids was 0.6336 ~2.2721 mg /g of 10 kinds of processing technologies,between which there were nine kinds of processing technologies beyong the Chinese Pharmacopoeia requirement of 0.7 mg /g,while the total alkaloids content was 7.8019 ~ 11.2078 mg /g.Conclusion: The best method for reducing poison is high-pressure steaming,whose content of diester alkaloids can reach the Chinese Pharmacopoeia requirement after treatment of wet autoclaved products,with higher content of total alkaloids.It is simple,controllable,and suitable for processing of Aconiti Kusnezoffii Radix.