The immunoglobulins and glycosaminoglycans analysis of fluid aspirated from postoperative maxillary cysts

We measured total protein (TP) and immunoglobulin levels in cyst fluid taken from 19 patients who had postoperative maxillary cysts (POMC). In the 19 cases, TP, IgG, IgA, and IgM levels were 7990±3558 (mean±1 SD), 1890±1085, 648±1085, and 664±1489mg/dl, respectively. Immunoglobulin correlations for IgG and IgA, IgG and IgM, and IgA and IgM were 0.706, 0.654, and 0.951, respectively. Using cellulose acetate membrane electrophoresis, we also examined the glycosaminoglycan fractions in POMC fluid. Four (21.1%) of the 19 samples showed a separation-impossible fraction of glycosaminoglycans, while 15 samples (78.9%) showed a separation-possible fraction. By digestion with pronase, most samples could be separated into 3 bands: an unknown band, a hyaluronic acid band, and a chondroitin sulfate A band (CSA). The unknown bands of samples having a separation-impossible fraction and of those having a separation-possible fraction were 32.4±3.5% (mean±1 SD) and 58.4±9.6%. The hyaluronic acid bands in the two fractions were 56.0±12.9% and 30.7±8.1%, and the CSA bands were 5.7±8.0% and 8.7±4.3%. On the unknown and CSA bands, samples having a separation-possible fraction were higher than those having a separation-impossible fraction. On the hyaluronic acid band, samples having a separation-impossible fraction were higher than those having a separation-possible fraction, but there were no significant differences.