Study on deficient Se mouse with viral myocarditis and apoptosis

Objective The relationship between deficient Se mouse with viral myocarditis and apoptosis had been studied in this paper.Methods Male BALB/C mice (5-week-old) were randomly divided into I experimental group, Ⅱ control group and were fed Se-deficient and Se-normal food respectively. After 5 weeks, mice in groupⅠ were intraperitoneally injected CVB 3m10 33 TCD 50 by the dosage of 0.1ml as well as mice in groupⅡ with the same dosage 1640 liquor.Results Pathological changes of myocardial issues were observed through LT and ET after mice were killed 7 days later original injection.The results showed that ,by LT, detection rates of pathological change between mice in groupⅠ(75%) and group Ⅱ(35%) are obviously different (χ 2 check P0.05). By means of TDT/dUTP nick-end labelling method (TUNEL), apoptosis cells and expression of C-myc and TGF-β 1 could been found in myocardium of Se-deficient diet feeding mice and regular diet feeding mice. No or less apoptosis could been observed in the regions where BCL-2 expressed highly.Conclusions Results of this project showed that Se-deficiency could increase apoptosis high expression in myocardial tissues to CVB 3m attack virus. Se-deficiency could increase C-myc 、TGF-β 1 high expression.