Method for preparing grease and protein from oil seed kernels by aqueous enzymatic method

The invention relates to a method for preparing grease and protein from oil seed kernels by an aqueous enzymatic method and belongs to the technical field of the application of the biotechnology to the grain and oil food processing. The method for preparing grease and protein from the oil seed kernels by the aqueous enzymatic method is characterized by comprising the following steps of 1) oil preparation through squeezing; 2) crushing; and 3) enzymolysis and drying: water and complex enzyme are added into treated squeezed cakes, the pH is regulated to the pH value and the temperature applicable to carbohydrase, and the enzymolysis is carried out for 15 to 90 minutes; the pH is regulated to the pH value and the temperature applicable to the protease, and the enzymolysis is carried out for 15 to 90 minutes; the pH is regulated to 9.0 after the enzyme reaction, and the stirring alkali extraction is carried out for 10 to 60 minutes at room temperature; boiled oil and protein water solution are separated through centrifugation; and the boiled oil is subjected to vacuum drying at 100 to 120 DEG C, grease products are obtained, the protein water solution is subjected to spraying drying, and plant protein is obtained. The method has the characteristics of high oil yield, low water consumption and low energy consumption, and the additional value of oil processing products can be improved.