Effects of Experimental Conditions on Silver Nanoparticles' s Biosynthesis by Culture Supernatant of Penicillium chrysogenum

[Objective] The aim was to study the effects of experimental conditions on silver nanoparticles's biosynthesis by culture supernatant of Penicillium chrysogenum.[Method] The effects of initial silver nitrate concentration,pH value,visible-light emission and microwave irradiation on the reaction rate and size of synthetic silver nanoparticles were studied by using UV-vis spectra and transmission electron microscopy.[Result] The optimal initial silver nitrate concentration was 2 mmol/L;the reaction rate was increased with the increase of pH value in the reaction system,but the particle size was decreased;both visible-light emission and microwave irradiation could promote the synthesis of silver nanoparticles effectively.[Conclusion] This research provided a test basis for the realization of controllable synthesis of silver nanoparticles.