Isolation and purification of triterpenes in inonotus obliquus by Macroporous resins

This study aimed to develop the purification method of triterpenes in Inonotus obliquus by macroporous resins.The suitable macroporous resin was screened,the technological conditions of purification were studied with the adsorption rate and desorption rate of triterpenes were taken as evaluation indexes.And the optimum purification processing was obtained by the static adsorption and desorption tests.The results show that in five types of the experimented macroporous resins,AB-8 macroporous resin presents the best effect of separation.The optimum purification processing was as follows:the aqueous solution of crude extract of Inonotus obliquus at natural pH level with the volume of resin at 10 mL/g were absorbed by AB-8 resin with 12 h,and then washed with distilled water,30% ethanol and 70% ethanol in proper order at 12 h each.With the optimized technology,the adsorption rate and desorption rate of triterpenes were 93% and 80%.In conclusion,AB-8 resin can be applied to purify triterpenes in Inonotus obliquus.