Biomarkers of oxidative stress and antioxidants in severe asthma: A Prospective Case-Control Study.

Abstract Background Bronchial airway inflammation is the hallmark of asthma, which may be driven by an imbalance between oxidative stress and antioxidant defenses. Antioxidants deficiency may play a role, but this has remained unconfirmed. Objective To evaluate the oxidative stress burden and antioxidants defenses in patients with increasing asthma severity. Methods This prospective case-control study compared fractional exhaled nitric oxide (FeNO), exhaled breath condensate nitrite/nitrate (EBC-NOx), spirometry, and serum vitamins and trace elements among patients with and without asthma. Results Sixty participants were recruited (30 with severe asthma number; 23 women [76.7%]; mean age, 41.4 years; mean forced expiratory volume in 1 second [FEV 1 ], 2.2 L [72.2% predicted]; mean inhaled corticosteroid dosage, 2,540 μg/d; 18/30 [60%] receiving maintenance oral corticosteroids; 15 with mild asthma; all corticosteroids naive; 9 women [60%]; mean age, 34.6 years; mean FEV 1 , 3.48 L [100.5% predicted]; 15 healthy controls; 12 women [80%]; mean age, 37.6 years; and mean FEV 1 , 3.53 L [111.7% predicted]). The mean FeNO levels increased significantly with increasing asthma severity ( P  = .01), but the EBC-NOx levels did not change significantly ( P  = .90). Paradoxically, vitamin A and vitamin E increased with increased disease severity, with vitamin E levels increasing significantly ( P  = .07 and P P  = .37), zinc ( P  = .97), or selenium ( P  = .90). Conclusion FeNO but not EBC-NOx is increased significantly with asthma severity with no evidence of vitamins or trace elements deficiency in severe asthma. Impaired oxidative stress defenses in severe asthma may be driven by factors other than vitamins or trace elements deficiency.