Nephroprotective Effect of Aqueous Extract of Pimpinella anisum in Gentamicin Induced Nephrotoxicity in Wistar Rats
2018
Background: Pimpinella anisum known for its various medicinal properties is also a natural
antioxidant and a free radical scavenger with no documented evidence as a nephroprotective
agent. Objective: To evaluate the nephroprotective activity of aqueous extract of Pimpinella
anisum seeds in a rodent model of gentamicin induced nephrotoxicity. Materials and Methods:
Wistar albino rats of either sex, weighing 150–200 g was divided into 5 groups; normal saline,
gentamicin 80mg/kg, intraperitoneally for 8 days, aqueous extract of Pimpinella anisum seeds
at 1, 2, and 4g/kg, per oral for 8 days, the test extract administered 3 days prior and concurrently
with gentamicin for 5 days. Blood urea, serum creatinine, uric acid and blood urea
nitrogen analyses and microscopic examination of kidney were performed. Results: Gentamicin
treatment caused nephrotoxicity as evidenced by marked elevation in serum urea, serum
uric acid, serum creatinine and blood urea nitrogen (107.5±16.92mg/dl, 0.8±0.09 mg/dl,
3.05±0.29 mg/dl, 47.8±9.07 mg/dl) respectively when compared to the saline treated groups.
Co-administration of Pimpinella anisum extract with gentamicin decreased the rise in these
parameters in a dose dependent manner. Histopathological analysis revealed epithelial loss
with intense granular degeneration in gentamicin treated rats, whereas aqueous extract of
Pimpinella anisum mitigated the severity of gentamicin-induced renal damage. Conclusion:
To conclude, our data suggest that aqueous extract of Pimpinella anisum exhibits renoprotective
effect in gentamicin induced renal damage and further studies on its mechanism
of action are warranted.
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