Crenalkalicoccus roseus gen. nov., sp. nov., a thermophilic bacterium isolated from alkaline hot springs.
2016
Two closely related thermophilic bacterial strains, designated YIM 78023T and YIM 78058, were isolated from samples collected from two alkaline hot springs in Tengchong county, Yunnan province, south-west China. The novel isolates were Gram-stain-negative, non-motile, aerobic ovoid- to coccoid-shaped and non-spore-forming. Strain YIM 78023T grew at 20–60 oC and pH 6.0–9.0 with optimal growth observed at 40–50 oC and pH 8.0, while strain YIM 78058 grew at 25–60 oC and pH 6.0–10.0 with optimal growth at 45–50 oC and pH 8.0. Phylogenetic analysis based on 16S rRNA gene sequences affiliated these two isolates within the family
Acetobacteraceae
with high sequence similarities to members of the genera
Roseomonas
and
Belnapia
(all sequence similarities <94.5 %). In addition to the above two genera, these strains also clustered with the genera
Craurococcus
and
Paracraurococcus
(having sequence similarities <93.3 %) in the phylogenetic tree, but with a distinct lineage within the family
Acetobacteraceae
. The major ubiquinone was Q-10 and the major fatty acids observed were C18:1
ω7c, summed feature 4 and C16:0. The genomic DNA G+C contents observed for strains YIM 78023T and YIM 78058 were 74.3 and 74.0 mol%, respectively. Morphological, phylogenetic and chemotaxonomic results suggest that strains YIM 78023T and YIM 78058 are representatives of a novel species of a new genus within the family
Acetobacteraceae
, for which the name Crenalkalicoccus roseus gen. nov., sp. nov. is proposed. The type strain of Crenalkalicoccus roseus is YIM 78023T (=JCM 19657T=KACC 17825T).
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