Effects of Stable Transfection of Exogenous Interferon-β Gene on Apoptosis of SHG-44 Glioma Cell Line
2005
Objective To investigate the effects of stable transfection of interferonβ (IFN-β) gene on the apoptosis of human gliomas cells in order to explore new methods of gene therapy for glioma. Methods Eukaryotic expression vector (pSV2IFNβ) for IFN-β was transduced into SHG44 glioma cell line by using liposome transfection. The stable transfection and expression of IFN-β in the SHG44 glioma cells were determined by flow cytometry and immunoflurescence method. The cell apoptosis was observed by Hoechst staning and an electron microscope. Results IFN-β gene was successfully transfected into and expressed in SHG44 glioma cell. IFN-β induced SHG44 glioma cell apoptosis. Conclusion That IFN-β gene can induce SHG44 glioma cell apoptosis provides an elementary basis for the treatment of glioma by using IFN-β gene.[
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