Cryogenic synchrotron radiation X-ray fluorescence analysis of biological model organisms using a state-of-the-art cryochamber at P06, PETRA III

2D sweep scan Incident energy: 10.5 keV Scan dimensions: 205 (0.5 μm) 165 (0.5 μm) Exposure time: 0.55 s/point Chlamydomonas reinhardtii Prorocentrum lima Conclusions and future prospects The cryochamber is a valuable sample environment for the analysis of microalgae under cryogenic conditions. Zn and Cu are clearly accumulated within the exposed algae. Zn is homogeneously distributed, in contrast to Cu that is mainly accumulated at the borders of P. lima. Copper is also present in areas that so far cannot be explained (leaching?). The P, Ca and Fe distributions give a good indication of the overall algae distribution. However, the P distribution indicates sample pile-up of C. reinhardtii on top of P. lima. In future experiments, sample pile-up should be avoided by working with lower sample concentrations or by investigating both algae separately. Quantification will be performed after the measurement of a suitable standard reference material (e.g., NIST 1755c – Bovine Liver). 205 0.5 μm 1 6 5 0 .5 μ m C. reinhardtii on top of P. lima