Indigenous bacterial community and function in phenanthrene-polluted coastal wetlands: Potential for phenanthrene degradation and relation with soil properties

Abstract The Yellow River Delta, adjacent to Shengli Oilfield, has a potential risk of petroleum pollution. In this study, soil samples were collected from phenanthrene (PHE)-polluted (adjacent to abandoned oil well, Zone D) and non-polluted (far away from abandoned oil well, Zone E) coastal wetlands. The influence of PHE pollution on indigenous bacterial community and function, and their relationship with soil characteristics were investigated. The levels of PHE, salinity and NH4+-N were higher in Zone D than in Zone E. PHE-degrading bacteria Achromobacter and Acinetobacter were mainly distributed in Zone E, whereas Halomonas, Marinobacter, and Roseovarius were highly abundant in Zone D. Halomonas and Marinobacter had the potential for denitrification and could achieve PHE degradation through mutual cooperation. PHE pollution could increase the abundance of functional bacteria but reduce the diversity of microbial community. PHE and salinity played key roles in shaping microbial community structure and function. High PHE level inhibited microbial metabolism but stimulated self-protection potential. PHE aerobic degradation associated with the catechol and phthalic acid pathways was found in Zone D, whereas the catechol pathway dominated in Zone E. Interestingly, PHE anaerobic degradation with nitrate reduction also dominated in Zone D, whereas the process coupled with multiple electron acceptors co-existed in Zone E, which was associated with tidal seawater carrying nutrients. This study illustrated the importance of comprehensive consideration of microbial community structure and function under PHE pollution, suggesting indigenous microorganisms as potential microbial consortium for bioremediation in coastal wetlands.