699 QUANTITATIVE MICROIMMUNOASSAY FOR IMMUNO-GLOBULIN CLASS SPECIFIC HUMAN ANTIBODIES

In order to evaluate antibody responses in immunodeficient patients, we have developed a modification of the enzyme linked immunosorbent assay (ELISA) which is 1) sensitive, 2) specific for immunoglobulin class, 3) applicable to numerous antigens, and 4) quantifiable. The method requires sequential addition to microtiter wells of 1) antigen, 2) serially diluted serum, 3) anti immunoglobulin-alkaline phosphatase conjugate, and 4) synthetic enzyme substrate.