Folding mechanism of mutant human lysozyme C77/95A with increased secretion efficiency in yeast

clarify the molecular features of C77195A and the reason for its increased secretion in yeast, the stabilities of the mutant C77/96A and the wild-type proteins were examined by guanidine hydrochloride denaturation, and the unfolding-refolding kinetics were determined from circular dichroism and fluores- cence stopped-flow measurements. Equilibrium exper- iments showed that the