[Clinical significance and detection of the expression of CD25- CD127- on CD4+ T cells in peripheral blood in patients with hepatitis B].

Objective To investigate the clinical significance and detection of the expression of CD25 - CD127 - on CD4 + T cells in peripheral blood in patients with hepatitis B.Methods The expression of CD25- CD127- on CD4 + T cells were measured by using flow cytometry in 53 patients with chronic hepatitis B,53 carrier with hepatitis B virus and 26 healthy blood donors,and follow up 20 patients with HBV-DNA positive treated with interferon.Results ① Compared with healthy controls,the expression of CD25 - CD127 - on CD4 + T cells in patients and carrier with hepatitis B virus were lower( Q =4.559,P ＜0.05 ; Q =6.230,P ＜ 0.05 ).②The expression of CD25 - CD127 - on CD4 + T cells in patients with HBV-DNA positive( n =77 ) was lower than that of negative ( n =29 ) ( t =2.290,P =0.024 ).③Compared with the prior treatment,the expression of CD25 - CD127 - on CD4 + T cells in patients with B hepatitis were lower after interferon treated with 12 weeks(t =2.469,P =0.024).Conclusion It suggested that the CD25-CD127- expression on CD4 + T cells correlated with viral infections and cleared,exogenous interferon could decrease CD25 - CD127 - expression on CD4 + T cells. Key words: Hepatitis B virus;  T-lymphocyes ;  Receptors, interleukin-2 ;  Receptors,interleukin-7 ;  Flow cytometry