Parámetros óptimos en la transformación de embriones somáticos de papaya empleando una pistola de genes de baja presión

Genetic improvement of papaya ( Carica papaya L.) is of great importance for its commercialization in the world. In this sense biolistic, as a genetic transformation method constitutes an alternative. Using a low pressure gene gun, papaya somatic embryos were bombarded with the plasmid pCAMBIA3301. The assays were evaluated by histochemical analysis of the gus gene expression. Optimal genetic transformation conditions were obtained with a maximum efficiency of 77.8 blue points per 100 mg of somatic embryos. Key words: β-glucuronidase, bombardment, microparticles