A linearization method for low catalytic activity enzyme kinetic analysis

Abstract A kinetic analysis was made and a linear plot based on the general rate equation derived by Laidler [Can. J. Chem. 33, 1614–1624] is proposed. This linearization method allows determining the kinetic parameters ( K m , k cat ) and [E] 0 for enzymes with low catalytic activity. The method was applied to chloroperoxidase from Caldariomyces fumago [EC 1.11.1.10], whose kinetic parameters K m app , k cat app , and [E] 0 with monochlorodimedone as substrate, were obtained by using the linearization plot and the V max value (calculated by Eadie–Hofstee plot). This plot could also be useful to the study of abenzyme kinetics provided the concentration of the latter is either higher or equal than K m value.