Polymorphism R25P in the gene encoding transforming growth factor‐beta (TGF‐β1) is a newly identified risk factor for proliferative diabetic retinopathy
2002
Association of the genetic polymorphisms in the promoter region
and the signal peptide sequence of the transforming growth
factor-beta (TGF-s1) gene with proliferative diabetic
retinopathy (PDR) in patients with non-insulin dependent
diabetes mellitus (NIDDM) were studied. A total of 245
Caucasian subjects comprised the two groups: NIDDM patients
with PDR (n=73) and NIDDM patients without PDR (n=172). Allele
frequencies of common TGF-s1 polymorphisms (at positions
-988C/A, -800G/A, -509C/T, +869T/C (L10P) and +915G/C (R25P))
were determined by polymerase chain reaction based methodology.
All polymorphisms were in strong linkage disequilibrium
(P<10-2). Significantly higher frequencies of both the L allele
and the R allele of the signal sequence polymorphisms in PDR
subjects were found (after a correction for multiple
comparisons Pcorr<10-2 and Pcorr<10-4, respectively).
Calculated odds ratios for the LL and RR genotypes were 2.89
(95% CI, 1.6-5.1) and 19.73 (95% CI, 2.6-146.8), respectively.
No significant differences between groups were found for the
-800G/A and - 509C/T polymorphisms. The - 988A allele was not
represented in our sample. Multiple logistic regression
identified age, diabetes duration and R25P polymorphism as a
significant predictors (P=0.002, P=0.000003, P= 0.007,
respectively). The frequencies of genotype combinations of the
-800G/A, -509C/T, L10P and R25P TGF-s1 polymorphisms were
significantly different between the PDR and non-PDR groups
(?2=37.83, df=20, P<10-2). Frequency of haplotype consisting of
majority alleles was found significantly associated with PDR
(P<0.03). The presented data indicate that the R25P
polymorphisms in the TGF-s1 gene could be regarded as a strong
genetic risk factor for PDR.
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