Induction of Conjugation in Tetrahymena

: INTRODUCTIONThe method to induce Tetrahymena conjugation described below is modified from that of Martindale et al. (1982). Mature Tetrahymena cells of opposite mating types are starved under appropriate salt conditions. The mating types are then combined to costimulate through cell-cell interaction. Loose pairs and then firm, irreversible pairs of cells of opposite mating types form. This method consistently results in a high percentage of pairing (usually greater than 80%) and good synchrony.