[High performance preparation and structural confirmation of lignans from Schisandrae chinensis fructus by using HSCCC combined with ESI-MSn method].

High-speed counter-current chromatography(HSCCC) was used to high performance separate and prepare lignans from Schisandrae chinensis fructus. The solvent system is composed of n-hexane- ethyl acetate- methanol- water(9∶1∶5∶5) and n-hexane- ethyl acetate- methanol- water(9∶1∶9∶5), speed is at 900 r·min-1, and flow rate is at 2.0 mL·min-1. Five fractions from Schisandrae chinensis fructus extract were separated and prepared with one HSCCC process. They were identified as schisandrin, gomisin J, schisandrol B, schisantherin A and deoxyschizandrin by electrospray ionization-multiple tandem mass spectrometry(ESI-MSn), respectively. Their contents were obtained in 98.74%, 94.32%, 99.53%, 94.23% and 98.68% by ultra high performance liquid chromatography(UPLC), separately. The rapid and simple method can be applied for the preparation of lignans from Schisandrae chinensis fructus.