Histological Effect of Oral Administration of Ccl4-Induced Stomach and Duodenum Damage and the Protective Effect of Nigella Sativa in Adult Wistar Rats

Nigella sativa is a herbal plant commonly used for the treatment of several diseases including gastric ones. This study investigates the histological effects of cool extraction of Nigella sativa oil against CCl4- induced stomach and the duodenum damage in adult Wistar rats of both sexes. 35 adult Wistar rats of both sexes were equally divided into 5 groups (5 rats). Group 1 rats were administered normal Saline (volume per body weight) orally. Group 2 rats were administered olive oil 4ml/kg body weight orally. Group 3 rats were administered 4ml/kg body weight of N. sativa oil orally for 2 weeks. Group 4 rats were administered 4ml/kg body weight CCl4 (30% CCl4 in 70% olive oil) orally. Group 5 rats were administered 4ml/kg body weight of N. sativa oil plus 4ml/kg body weight CCl4 (30% CCl4 in 70% olive oil) orally. Histopathological changes were observed. The stomach and the duodenum in group 1, 2 and 3 showed normal stomach and the duodenum architecture. There was a widespread degeneration and ulceration in the stomach and the duodenum in the epithelium of group 4 rats. N.sativa oil showed protective effect on the histological section of the stomach and the duodenum in groups 5. From the results it is suggested that cool extraction of Nigella sativa oil extract has the ability to protect the stomach and the duodenum histological damages, possibly through antioxidant effects