Age-related changes of bone ultrasound measurements and metabolic indicators in the young horse

Abstract A longitudinal field study was performed in order to characterize age-related changes of ultrasound measurements, biochemical markers, growth factors and other metabolic variables related with bone metabolism in the young horse. Twenty-seven Lusitano foals (11 colts, 16 fillies), born and raised on four stud-farms, were periodically assessed from birth to 36 months of age. Quantitative ultrasound measurements were performed on the mid-section of the right third metacarpal bone (dorsal and lateral regions) and blood samples were collected for determination of osteocalcin (Oc), bone alkaline phosphatase (BALP), insulin-like growth factor I (IGF-I), leptin, insulin, glucose, parathyroid hormone (PTH), calcium, phosphorus and magnesium plasma concentrations. Data were analyzed using a repeated measures model that includes the fixed effects of time (days of age), time × time, sex, foaling season, their interactions, and the farm as a random effect. Speed of sound values increased with age ( P P P P r  = −0.098; P