The empirical study on E6-AP with carcinoma of prostate cell migration and infiltration
2010
Objective:The carcinoma of prostate is a prostate gland familiar malignant tumor.It transfers gradually People pay attention to its migration and infiltration.This text mainly studies the relativity that the E6-AP with carcinoma of prostate cell migration and infiltration.For inquirying into the gradually transfer of the carcinoma of prostate mechanism to provide an experiment.Methods:Apply the electricity boring method turns line of PrevTet-off-in,adjusting to control the quality grain and the PrevTRE2-flag-E6 AP reorganization plasmid ducting prostate gland cancer cell(Lncap),and build up the prostate gland cancer of the stability expression E6-AP.Apply the MTT experiment an examination cell to propagate.The applied Matrigel Invasion Chamber examination tumor cell moves gradually characteristic.Results:The MTT shows the stability turns the cell that the prostate gland cancer cell of dye the expression E6-AP ratio didn't turn to dye to have as a result stronger propagate an activity.The stability that expresses E6-AP turns to dye cell to have stronger crawl along and adhere to an ability.Conclusion:The E6-AP albumen and carcinoma of prostate cell migration and infiltration is closely related
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