Anti-diabetic activity peptides from albumin against α-glucosidase and α-amylase

Abstract The objectives of this study were to identify novel peptides from albumin, and to evaluate and validate the anti-diabetic activity of peptides against α-glucosidase and α-amylase. In the research, albumin hydrolysate was purified and identified, tandem MS was adapted to characterise the amino acid sequences of peptides from the hydrolysate. In addition, anti-diabetic effects of the peptides with α-glucosidase and α-amylase inhibitory activity have been performed. The present work found eight novel peptides from albumin. Results also suggested that peptide KLPGF had α-glucosidase inhibitory activity with an IC 50 of 59.5 ± 5.7 μmol l −1 and α-amylase inhibitory activity with an IC 50 of 120.0 ± 4.0 μmol l −1 . In conclusion, the results revealed that the peptide KLPGF was a potential anti-diabetic inhibitor.