New electrochemical DNA sensor based on nanoflowers of Cu3(PO4)2-BSA-GO for hepatitis B virus DNA detection

Abstract Hepatitis B virus (HBV) DNA is an important biomarker for chronic HBV infection. To date, the majority of HBV biosensors have only focused on five basic factors (HBsAg, anti-HBs, HBeAg, anti-HBs, and anti-HBc), thus a sensitive, low-cost, and stable electrochemical sensor to measure the concentration of HBV-DNA remains needed. We present here a sensitive electrochemical DNA sensor that employs new nanoflower particles, gold nanoparticles, and two surface-immobilized aptamers to amplify the signal. The complex biosensor layers provide not only sufficient binding points, but also an adaptive framework for signal amplification and biocompatibility. The electrochemical DNA sensor exhibits a wide linear detection range from 1.10 × 103 to 1.21 × 105 copies/mL and a detection limit of 1100 copies/mL for HBV-DNA. The excellent performance in hepatitis B virus propagation detection suggests that the proposed electrochemical DNA sensor has great application potential in clinical diagnosis.