Determination of Paeoniflorin in Xiaoyao Pills by HPLC

OBJECTIVE To establish a method for determination paevoniflorin in Xiaoyao Pills.METHODS HPLC was used the amalysis stical columa was kromasil KR100 5C 18 and the mobile phase was acetonitrile water(20∶80)at a flow rate of 1 0mL·min -1 , the UV detection wavelength was 230nm.RESULTS In the scope of 0 222～1 77μg the contents of paeoniflorin had good linear relationship with their peak area (r=0 99997,n=6).The average recoveries were 97 9%,RSD=0 8%(n=6).CONCLUSION The results showed that the method was rapid,simpie excellent precision.