Analysis of the genetic diversity of six Walnut Seeding-Species in XinJiang by RAPD

In order to research the diversity of six walnut seeding species which belonged to XinJiang , the leaves of 6 female and F1 generations of procecious mature walnut were used to extract genomic DNA. 7 single primers were selected from 55 random primers which have been selected for RAPD. RAPD amplification was used for studies on genetic diversity and SPSS was used to calculate heredity distance, and the dendrogram was constructed by UPGMA base on heredity distance. There were 81 products were used to RAPD analysis and 63(77.8%) bands were polymorphic loci, every primer amplified 9 bands in average. The results showed that the female parents of six species were classified into three groups, and the generation of six groups could be classified into two groups, but the heredity distance was large. There were gene flow among different females and F1 generations, the result will gave us some information for research diversity of walnut in future.