The development of methods for the detection of Salmonella in chicken by a combination of immunomagnetic separation and polymerase chain reactions

Micro- and nano-immunomagnetic beads (MIMBs and NIMBs) used for immunomagnetic separation (IMS) with Polymerase Chain Reaction (PCR) were studied for the rapid detection of Salmonella. The capture efficiency of the two different IMBs was evaluated by a conventional plate counting method, and the binding pattern was studied by scanning electron microscopy. The specificity of the IMBs was tested with Salmonella, Shigella flexneri, Enterhemorrhagic Escherichia coli O157:H7(EHEC O157:H7) and Listeria monocytogenes. By comparing the pre-enrichment-IMS and the IMS-enrichment steps with a 5.5 h enrichment time, this study developed a rapid and sensitive method for the detection of Salmonella in chicken. The method was implemented by IMS-enrichment and PCR with MIMBs and NIMBs, with a total analysis time of 8 h. We showed that the method was sensitive based on NIMBs with a detection limit for Salmonella of 10° CFU in 25 g of chicken. This article is protected by copyright. All rights reserved