插天山羊耳蒜（蘭科植物）無菌播種與小苗培育

Liparis sootenzanensis Fukuyama is an endemic orchid in Taiwan and it's habitat around the islands. This species has the potential as ornamental plant in the coming future with the big flower and handsome plant sharp among the genus Liparis. In this study, we have established the in vitro seedling culture procedure. First, we observed the seed on microscope with linear morphology with 91% air space inner the seed, and considered to be a terrestrial species of Liparis. The superior quality seeds has 93.3% embryo rate and the 74.7%-80.3% seed germination rate. The mature seed sown in medium containing 1/4 MS basic salts, with organics supplement (peptone, potato powder and coconut water), activated charcoal. And the young seedlings were transferred to 1/2 MS basic salts with organics and activated charcoal to obtain best performance of plant height, leaf number, pseudobulb width and root length. This study has established reliable procedure for in vitro seed germination and seedling development of Liparis sootenzanensis Fukuyama, and could be used in germplasm preservation, repopulation and ornamental plant in the future.