Identification of Listeria species by a semi-nested polymerase chain reaction

Abstract Three primers derived from the iap gene were used to distinguish Listeria monocytogenes from L. innocus and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welshimeri .