Lymphocystis disease virus persists in the epidermal tissues of olive flounder, Paralichthys olivaceus (Temminch & Schlegel), at low temperatures.

Olive flounder artificially infected with lymphocystis disease virus (LCDV) were reared at 10, 20 and 30 � C for 60 days, to compare LCD-incidence. In the fish reared at 20 � C, lymphocystis cells appeared on the skin and fins at 35 days post-challenge, and the cumulative LCD-incidence was 80% at 60 days. High levels of LCDV, with a mean polymerase chain reaction (PCR) titre of 10 6 PCRUm g )1 tissue, were detected in the fins and skin of LCD-affected fish at 20 � C, but were not detected in the spleen, kidney, brain and intestinal tissues of these fish. No LCD clinical signs were observed in the fish reared at 10 � C and 30 � C; however, a low level of LCDV (10 3 PCR-U mg )1 tissue) was detected in the fins and skin of these fish. By increasing the rearing temperature from 10 to 20 � C, lymphocystis clusters appeared on the skin and fins of the fish with no previous LCD clinical signs within 33 days after the temperature change. It was shown that permissive cells for LCDV infection exist in the epidermis of olive flounder. At low temperatures, small amounts of LCDV were able to persist over a period extended for a further 45 days in the fish epidermis, even though the fish showed no LCD clinical signs. The optimum growth temperature of LCDV is near 20 � C.