In vitro toxicity evaluation of silver soldering, electrical resistance, and laser welding of orthodontic wires

SUMMARY The long-term effects of orthodontic appliances in the oral environment and the subsequent leaching of metals are relatively unknown. A method for determining the effects of various types of soldering and welding, both of which in turn could lead to leaching of metal ions, on the growth of osteoblasts, fi broblasts, and oral keratinocytes in vitro , is proposed. The effects of cell behaviour of metal wires on osteoblast differentiation, expressed by alkaline phosphatase (ALP) activity; on fi broblast proliferation, assayed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenil)2H-tetrazolium – phenazine ethosulphate method; and on keratinocyte viability and migration on the wires, observed by scanning electron microscopy (SEM), were tested. Two types of commercially available wires normally used for orthodontic appliances, with a similar chemical composition (iron, carbon, silicon, chromium, molybdenum, phosphorus, sulphur, vanadium, and nitrogen) but differing in nickel and manganese content, were examined, as well as the joints obtained by electrical resistance welding, traditional soldering, and laser welding. Nickel and chromium, known as possible toxic metals, were also examined using pure nickel- and chromium-plated titanium wires. Segments of each wire, cut into different lengths, were added to each well in which the cells were grown to confl uence. The high nickel and chromium content of orthodontic wires damaged both osteoblasts and fi broblasts, but did not affect keratinocytes. Chromium strongly affected fi broblast growth. The joint produced by electrical resistance welding was well tolerated by both osteoblasts and fi broblasts, whereas traditional soldering caused a signifi cant ( P < 0.05) decrease in both osteoblast ALP activity and fi broblast viability, and prevented the growth of keratinocytes in vitro . Laser welding was the only joining process well tolerated by all tested cells.