Biological Applications of Surface Plasmon Resonance Imaging

This chapter will deal with versatile biochips. The presented biochips are prepared by the microarray functionalization of gold surface from millimetric to micrometric scale via different electrochemical approaches. Interactions occurring on these functionalized surfaces are monitored using surface plasmon resonance imaging approaches. Different size of spots including hyphenated techniques will be used to study biological interactions from molecular (i.e., DNA, proteins (toxins), and sugars) to complex objects such as living cells. Applications of this approach will be illustrated by examples from different biological fields including DNA hybridization studies, antibody screening in patients’ samples, study of oligosaccharides–proteins interactions, and lymphocytes characterization and sorting. For each topic, optimized chemistry and optical developments will be specified.