Effect of flaxseed Lignans concentrate loaded Transfersome on oxidative stress in Caenorhabditis elegans

In continuation with our previous study, this experiment was carried out to determine the effect of the transferosome (TRF) loaded with flaxseed lignans concentrate on the lifespan and oxidative status of C. elegans under induction of oxidative stress by H2O2. Transfersomal formulation prepared using bio surfactant sophorolipid and transferosomes were tagged with FITC to confirm the penetration through nematode cuticle hypothesis was tested. Lifespan was determined by counting the number of surviving nematodes daily under a Lumascope microscope after treatment with TRF. The evaluated aging oxidative markers included lipofuscin, which was measured using a fluorescent microscope. In our study, 0.2 mg/ml TRF was found to be the optimum concentration to increase the mean lifespan of C. elegans, the accumulation of the age marker lipofuscin, which increased with hydrogen peroxide exposure, was decreased with upon treatment with TRF. The TRF enhanced the lifespan C. elegans and reduced the accu ulation of lipofuscin trend remains matches with the non-encapsulated flaxseed lignans concentrate. Further, study confirms that possibility of penetration of FITC tagged transfersomes via cuticle of nematode was confirmed by imaging.